Discover the elements that power NIOX® devices.
NIOX® was founded by the scientists that originally discovered NO's link to airway inflammation. Our technology has always been designed with two things in mind: accuracy and ease of use.
Scroll down to explore the testing journey with NIOX®
To begin with, the healthcare professional will pass a new FeNO Protect Patient Filter® to the patient, instructing them to twist it onto the NIOX® Breathing Handle.
≥99.99% Bacterial Filtration - ≥99.97% Viral Filtration1

Next the patient will be asked to inhale. This is important as the NIOX® Breathing Handle contains a NIOX NeutroNO® Scrubber that removes ambient NO on inhalation, preventing contamination to measurements.
Ambient NO can vary from 2 to 500ppb!2 Consider the potential impact on FeNO if it isn’t removed!

Following our NIOX Accessible Aids®, the patient will exhale once they have inhaled. Exhalation will either be for 10 seconds or 6 seconds, depending on the test mode selected (adult or child).
NIOX® captures the perfect 50 ml / second
NIOX® devices keep just the last three seconds of the 10-second exhalation to ensure that only breath from lower in the airways is tested.
During exhalation, our unique FeNO Dry® Technology removes excess humidity from the breath sample.

By the time the air sample enters the NIOX Aerocore®, where it is stored for analysis, it has been cleansed from ambient NO, viruses and bacteria, and excess humidity.
Only the sample from the patient remains

Next, the device moves into the analysis phase, following the below steps.
Next, the sample in the NIOX Aerocore® passes over our industry-leading NIOX® Sensor, where the analysis takes place.
Sample analysis takes 30 seconds.
NIOX® also takes a ‘zero gas’ baseline measurement immediately after the patient’s test to further ensure that FeNO values are accurate and reliable.3,4
Baseline analysis takes an additional 30 seconds.
The NIOX PurePrecision™ algorithm checks the linear relationship between sensor signal and gas concentration, to ensure accurate results.4
Our algorithm is constantly working throughout analysis

At NIOX®, it is not all about having the best technology and customer experience, we pride ourselves in offering a simple and easy to use platform for both our customers and patients.
Testing FeNO with NIOX® is effortless. Just one test for one accurate result, and with Flow Rate Control™, patients can never fail the test.4,5

Our dedicated operating system, nioxOS® has a variety of additional features to make NIOX® simple and easy to use.1,3 At the heart of this is our NIOX Accessible Aids™, with four engaging animations paired with audio cues to guide patients to a successful test.
Discover nioxOS®
That’s right, NIOX® devices perform steps one to five in just 75 seconds, which is quicker than making a cup of coffee!
We are very proud of our unique FeNO testing technology. We understand that thousands of healthcare professionals rely on our devices to guide treatment decisions and amend therapies.7 So, we ensure that all of our products are accurate, reliable and easy to use.3,4,8
Request a quote for a device today with our simple and easy to use quotation tool. Used by thousands of healthcare professionals, NIOX® is the global technology of choice for FeNO testing.6,7
Request a QuoteAt NIOX® we are passionate about providing excellent support and training.
1. NIOX®. Technical Equivalence of NIOX PRO® and NIOX VERO® in Fractional Exhaled Nitric Oxide (FeNO) Measurement Whitepaper. 2025.
2. Clapp L et al. Analysis of the relationship between ambient levels of O3, NO2 and NO as a function of NOx in the UK. Atmos Environ. 2001;35(36):6391-6405.
3. Alving K et al. Validation of a new portable exhaled nitric oxide analyzer, NIOX VERO®: randomized studies in asthma. Pulm Ther. 2017;3:207-18.
4. Carroll WD. NIOX VERO: Individualized Asthma Management in Clinical Practice. Pulmonary Therapy. 2016;2:171-88.
5. Hanania NA et al. Measurement of fractional exhaled nitric oxide in real-world clinical practice alters asthma treatment decisions. Ann Allergy Asthma Immunol. 2018;120(4):414-418.
6. NIOX®. Data on file. MKT-DOF-007. 2026
7. NIOX®. Data on file. MKT-DOF-013. 2024.
8. Crater G et al. Successful fractional exhaled nitric oxide measurements with NIOX VERO using 6-and 10-s exhalation modes in children with asthma aged 4–10 years: a randomized study. Pulm Ther. 2017;3:139-48.